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  • Sample overview in the biobank

Article

Information about MoBa-samples in the biobank

Collection of flow charts, sample overview and number of total samples in biobank.

Collection of flow charts, sample overview and number of total samples in biobank.


Sampling timepoint and number of stored samples in the biobank for the MoBa cohort

Table 1 gives an overview of the sampling timepoint, number of received sample sets and the total number of individual samples divided by sample type. 

Table 1. Sampling timepoint and number of stored samples at the biobank for the MoBa study. The numbers are updated the 20th of September 2022.  

Collected from

Time of collection

Sample

Number of sample sets

Type of samples

Number of samples

Pregnant women

 

 

 

 

 

 

 

 

 

 

Week 17th -20th of pregnancy (K1 and K12*)

 

 

 

 

 

 

 

 

Blood and urine

 

 

 

 

 

 

 

 

 

 

100 585

 

 

 

 

 

 

 

 

 

 

Whole blood

96 498

Whole blood*

77 655

Plasma EDTA

98 956

Plasma EDTA*

69 956

DNA**

97 445

Urine*

9 737

Urine* (with chlorhexidine)

69 713

Father of the child

 

 

 

Week 17th -20th of partners pregnancy

 

 

Blood

 

 

 

73 521

 

 

 

Whole blood

72 166

Plasma EDTA

73 042

DNA**

71 677

Mother of the child

 

 

0-3 days after birth (K2)

 

 

Blood

 

 

 

87 366

 

 

 

Whole blood

85 161

Plasma EDTA

86 770

DNA**

80 678

Child

 

 

 

 

 

 

Day of birth

 

 

 

 

 

 

Blood from umbilical cord or capillary collection from newborn child

 

 

 

 

93 521

 

 

 

 


 

Whole blood

50 619

Plasma EDTA

91 829

DNA**

89 750

RNA Tempus

45 312

* Additional blood tubes and urine collected at week 17th - 20th of pregnancy, referred to as K12. Purpose use was measurement of environmental exposures during pregnancy.
** DNA concentration of approximately 100 ng/µl.

The biobank department at NIPH stores approximately 1.3 million individual samples from the MoBa participants. Further, the individual samples are alliquoted into several vials depending on the samples type. Figure 1 shows the percentage of the total amount of samples divided by sample types. 

figur 1
Figure 1. The percentage of the total amount of samples stored for the MoBa study, divided by samples type. Total stored samples from MoBa at the biobank at NIPH is almost 1.3 million..

Samples in the different projects and participant

Including the main cohort design, biological sample collection for specific projects within the MoBa cohort was conducted. These samples were collected for specific research purposes. Table 2 gives an overview of the different projects and participant, including the different sample types and number of collected samples. More information about the different research purposes of the projects can be found at the specific project/substudy pages.

 

Table 2. Overview of collection and number of stored samples for specific projects within the MoBa study. The numbers are updated the 20th of September 2022. 

Project  

Participants 

Number of sample sets

Sample type

Number of samples

ABC 

Child 

917

DNA

876

Whole blood, EDTA  

679

Plasma, EDTA  

904

RNA, Tempus

7

Mother

36

DNA

32

Whole blood, EDTA  

36

Plasma, EDTA  

33

Father

53

DNA

51

Whole blood, EDTA  

52

Plasma, EDTA  

49

Norwegian Human Environmental Biobank  

Child

671

Whole blood, EDTA

648

Plasma, EDTA  

604

Serum  

659

Urine  

669

RNA, Tempus

576

Mother

662

Whole blood, EDTA  

657

Plasma, EDTA  

657

Serum  

655

Urine  

660

RNA, Tempus

577

Father  

502

Whole blood, EDTA  

495

Plasma, EDTA  

497

Serum  

497

Urine  

500

RNA  

426

Women’s fertility  

Women age 18-24  

100

DNA  

83

Plasma, EDTA  

88

Whole blood, EDTA  

100

Urine, boric acid  

89

RNA, Tempus

100

MoBa Covid 19  

Child  

3 678

DNA  

3 620

Plasma, EDTA  

2 828

Mother

4 327

DNA  

4 260

Plasma, EDTA  

3 384

Father

3 529

DNA

3 482

Plasma, EDTA  

2 726

BraPust  

Child 7 - 10 years old  

454

DNA  

450

Plasma, EDTA  

447

Serum, SST

451

Language 8  

Child 8,5 - 9 years old

634

DNA from saliva  

634

Mothers

502

DNA from saliva  

502

Fathers

356

DNA from saliva  

356

The process from sample collection to biobank storage for all sampling timepoints

The flow charts (figure 2- 5) describe the process from sample collection to biobank storage for all sampling timepoints.

There are three main timepoints where biological material was collected in the MoBa study design. First was the collection from the pregnant women and the fathers of the child. This was done at week 17th – 20th of pregnancy at the planned ultrasound visit. A few years into the sample collection, the MoBa study received founding for a specific collection purpose. Two additional blood tubes and a urine sample was added to the collection from the pregnant women. The purpose was to measure environmental exposures during pregnancy. The whole blood was collected and stored anaerobe. These samples are referred to as K12. The fathers were invited to participate a few years after the collection from the mothers had begun.

The second timepoint was after birth. Biological material from the newborn child was collected from the umbilical cord or, in some cases, collected capillary from the child. A new sample collection from the mothers was also carried out 0-3 days after birth. 

The third was the collection of child primary teeth. This was carried out when the children were 6-7 years old. There are approximately 35 000 collected teeth. These are stored at the Department of Clinical Dentistry (IKO) at the University of Bergen. More information can be found at the MoBaTooth webpage.

Apart from the teeth, all biological material from the MoBa collection is stored at the biobank at Norwegian institute of public health (NIPH). Aliquots of whole blood, plasma and urine are stored at ultra-freezers and/or in the automatic storage unit at - 80 °C. Aliquots of DNA and one tube of whole blood are stored at freezers and/or in the automatic storage unit at – 20 °C. The RNA Tempus tubes collected from the child’s umbilical cord, or capillary from the newborn child, were first stored at -20 °C for 24 hours. They were later moved to - 80 °C ultra-freezers for long term storage.

One smaller tube of EDTA-blood, collected from the pregnant women, were frozen directly in the original tube. The RNA tempus tube, collected from the child, were also frozen in the original tube. Otherwise, the samples were divided to smaller aliquots before long term storage. After the DNA extraction from both whole blood and concentrated blood cells, the DNA was divided to aliquots of 100 ng/µl.

Figure 2. Flow chart of the process from MoBa sample collection to storage of the different specimen at the biobank. This chart shows the process for samples collected from the pregnant women taken at ultrasound at week 17th - 20th of pregnancy.  * Additional tubes added to the collection. Purpose use was measurement of environmental exposures during pregnancy. These samples are referred to as K12.
Figure 2. Flow chart of the process from MoBa sample collection to storage of the different specimen at the biobank. This chart shows the process for samples collected from the pregnant women taken at ultrasound at week 17th - 20th of pregnancy. * Additional tubes added to the collection. Purpose use was measurement of environmental exposures during pregnancy. These samples are referred to as K12.
Figure 3. Flow chart of the process from MoBa sample collection to storage of the different specimen at the biobank. This chart shows the process for samples collected from the fathers taken at partners ultrasound at week 17th - 20th of pregnancy.
Figure 3. Flow chart of the process from MoBa sample collection to storage of the different specimen at the biobank. This chart shows the process for samples collected from the fathers taken at partners ultrasound at week 17th - 20th of pregnancy.
Figure 4. Flow chart of the process from MoBa sample collection to storage of the different specimen at the biobank. This chart shows the process for samples collected from the mothers taken 0 – 3 days after birth.
Figure 4. Flow chart of the process from MoBa sample collection to storage of the different specimen at the biobank. This chart shows the process for samples collected from the mothers taken 0 – 3 days after birth.
Figure 5. Flow chart of the process from MoBa sample collection to storage of the different specimen at the biobank. This chart shows the process for samples collected from the child umbilical cord or capillary sample of the newborn child taken the day of birth  .
Figure 5. Flow chart of the process from MoBa sample collection to storage of the different specimen at the biobank. This chart shows the process for samples collected from the child umbilical cord or capillary sample of the newborn child taken the day of birth .