Whole-genome sequencing and antimicrobial resistance in Brucella melitensis from a Norwegian perspective
Project
|Updated
The aim of this study was to explore all Brucella melitensis isolates collected in Norway from 1999 to 2016 in relation to origin of infection and antimicrobial resistance patterns.
Summary
Brucellosis is a rarely encountered infection in Norway. The aim of this study was to explore all Brucella melitensis isolates collected in Norway from 1999 to 2016 in relation to origin of infection and antimicrobial resistance patterns. A total of 23 isolates were analysed by whole-genome sequencing and compared with selected sequences of B. melitensis available from NCBI. Additionally, SNP analysis in antibiotic resistance determining genes was performed. The majority belonged to the East Mediterranean clade (genotype II), while the remaining isolates belonged to the African clade (genotype III). These results indicate that human brucellosis in Norway is related to travels or migration from the Middle East, Asia or Africa, in accordance with results from Germany, Denmark and Sweden. Antibiotic susceptibility patterns were determined by broth microdilution method and/or gradient strip method. All isolates were susceptible for all tested antibiotics, except for rifampicin where phenotypical results indicated resistance or intermediate resistance in all isolates based on broth microdilution method, and in four isolates based on gradient strip testing. In contrast, screening of the rpoB gene did not reveal any mutations in the previously described rpoB “hot spot“regions related to rifampicin resistance, indicating overestimation of resistance based on phenotypical results.
Project leader
Tone Kristin Bjordal Johansen, Avdeling for smitte fra mat, vann og dyr, Norwegian Institute of Public Health
Project participants
Siri Laura Feruglio, Avdeling for smitte fra mat, vann og dyr, Norwegian Institute of Public Health
Veronica Klausmark Jensen, Avdeling for bakteriologi, Norwegian Institute of Public Health
Jon Bohlin, Avdeling for infeksjonsepidemiologi og modellering, Norwegian Institute of Public Health
Lonneke Scheffer, University of Oslo
Start
01.03.2016
End
31.05.2021
Status
Active
Approvals
Regional committees for medical and health research ethics
Project owner/ Project manager
Norwegian Institute of Public Health